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manual tirf illuminator  (Nikon)


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    Structured Review

    Nikon manual tirf illuminator
    Manual Tirf Illuminator, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 60152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/manual+tirf+illuminator/Objectives/pm41912541-525-8-12
    Average 99 stars, based on 60152 article reviews
    manual tirf illuminator - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Microscopy:

    Article Title: Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis
    Article Snippet: .. Live-cell STAR image acquisition was performed with a Nikon Ti-2 microscope equipped with a motorized stage, stage-top incubator to maintain 37 °C and 5% CO 2 (Tokai Hit, INUBG2SF-TIZB), ×60 1.49-NA objective, manual TIRF illuminator (Nikon, TI-LA-TIRF), 488 nm (Obis, 488-150 LS), and 647 nm (Obis, 1196627) excitation lasers, fiber coupling optics: fiber mount (Thorlabs, MBT621D), converging and directing the laser objective (Olympus, RMS10X), optical fiber (Thorlabs, P3-405BPM-FC-2), C-NSTORM QUAD 405/488/561/638 nm TIRF dichroic. .. Images were acquired with an Optosplit III (Cairn Research) image splitter with ET525/50 m and ET705/72 m emission filters (Chroma), and T562lpxtr-UF2 and T640lpxtr-UF2 dichroic mirrors to split the fluorescence emission onto separate regions of the ORCA-Flash 4.0 v3 scientific complementary metal-oxide-semiconductor camera (Hamamatsu).

    Article Title: Clathrin-associated carriers enable recycling through a kiss-and-run mechanism
    Article Snippet: The bottom surfaces of cells were imaged using a TIRF microscope built either on a Nikon Ti2-E microscope or a Nikon TiE microscope. .. The Nikon Ti2-E microscope was equipped with a manual TIRF Illuminator Unit (Nikon), a CFI Apochromat TIRF 100X objective (1.49 NA, Nikon), a Perfect Focus Unit (Nikon), a UNO Stage Top Incubator (Okolab), OBIS CellX lasers (405, 488, 561 and 637 nm, Coherent), W-VIEW GEMINI-2C Image Splitting Optics (Hamamatsu), and two EMCCD cameras (Evolve 512 Delta, Photometrics). .. The integrated 2ξ tube lens on the Nikon Ti2-E microscope was applied to achieve the final pixel size corresponding to 80 nm of the image.

    Article Title: Clathrin-associated carriers enable recycling through a kiss-and-run mechanism.
    Article Snippet: Endocytosis and recycling control the uptake and retrieval of various materials, including membrane proteins and lipids, in all eukaryotic cells.. These processes are crucial for cell growth, organization, function and environmental communication.. However, the mechanisms underlying efficient, fast endocytic recycling remain poorly understood.

    Article Title: Spatial organization of PI3K-PI(3,4,5)P3-AKT signaling by focal adhesions
    Article Snippet: After 24 hours, the cells were replaced with fresh medium containing 2 μg/mL puromycin (InvivoGen). .. The Nikon Ti2-E microscope was equipped with a manual TIRF Illuminator Unit (Nikon), a CFI Apochromat TIRF 100X objective (1.49 NA, Nikon), a Perfect Focus Unit (Nikon), an UNO Stage Top Incubator (Okolab), OBIS CellX lasers (405, 488, 561 and 637 nm, Coherent), the W-VIEW GEMINI-2C Image Splitting Optics (Hamamatsu), and two EMCCD cameras (Evolve 512 Delta, Photometrics). .. The bottom surfaces of cells were imaged using TIRF microscopy built on either a Nikon Ti2-E microscope or a Nikon TIE microscope.

    Article Title: Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis.
    Article Snippet: .. Live-cell STAR image acquisition was performed with a Nikon Ti-2 microscope equipped with a motorized stage, stage-top incubator to maintain 37 °C and 5% CO2 (Tokai Hit, INUBG2SF-TIZB), ×60 1.49-NA objective, manual TIRF illuminator (Nikon, TI-LA-TIRF), 488 nm (Obis, 488-150 LS), and 647 nm (Obis, 1196627) excitation lasers, fiber coupling optics: fiber mount (Thorlabs, MBT621D), converging and directing the laser objective (Olympus, RMS10X), optical fiber (Thorlabs, P3-405BPM-FC-2), C-NSTORM QUAD 405/488/561/ 638 nm TIRF dichroic. .. Images were acquired with an Optosplit III (Cairn Research) image splitter with ET525/50 m and ET705/72 m emission filters (Chroma), and T562lpxtr-UF2 and T640lpxtr-UF2 dichroic mirrors to split the fluorescence emission onto separate regions of the ORCA-Flash 4.0 v3 scientific complementary metal-oxide-semiconductor camera (Hamamatsu).

    other:

    Article Title: Lipid turnover and GEF recruitment collectively determine Rab5 recruitment and activation during the first step of early endosome formation.
    Article Snippet: AR TI CL E IN P RE SS Live-cell TIRF imaging and imaging analysis SUM159 cells plated overnight on single-well, 4-well, or 8-well confocal dishes (Cellvis) were imaged in phenol-free DMEM/F12 (Corning) containing 5% FBS and 20 mM HEPES.

    Article Title: Spatial organization of PI3K-PI(3,4,5)P 3 -AKT signaling by focal adhesions.
    Article Snippet: After 24 hours, the cells were replaced with fresh medium containing 2 mg/mL puromycin (InvivoGen).



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